Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning. PBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis.
Ligation independent cloning serial cloner free#
Predictive mutagenesis of ligation-independent cloning (LIC) vectors for protein expression and site-specific chemical conjugation.Īpplications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression.
Positive-selection and ligation-independent cloning vectors for large scale in planta expression for plant functional genomics.
High-throughput protein expression using a combination of ligation-independent cloning (LIC) and infrared fluorescent protein (IFP) detection. Medium-throughput production of recombinant human proteins: ligation-independent cloning.Ī ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes.Ĭreation and validation of a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells.Įnzyme treatment-free and ligation-independent cloning using caged primers in polymerase chain reactions.įunctional cell surface display and controlled secretion of diverse Agarolytic enzymes by Escherichia coli with a novel ligation-independent cloning vector based on the autotransporter YfaL.Ī high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.Ī versatile set of ligation-independent cloning vectors for functional studies in plants.Īn efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability. Ligation-independent cloning and self-cleaving intein as a tool for high-throughput protein purification. TupA: a tungstate binding protein in the periplasm of Desulfovibrio alaskensis G20.Īpplication of the ligation-independent cloning (LIC) method for rapid construction of a minigenome rescue system for Newcastle disease virus VG/GA strain.Ĭonstruction of new ligation-independent cloning vectors for the expression and purification of recombinant proteins in silkworms using BmNPV bacmid system.ĭNA fragments assembly based on nicking enzyme system. Recombinant expressed vector pET32a (+) S constructed by ligation independent cloning. Ligation-independent cloning (LIC) assembly of TALEN genes. High-throughput cloning, expression and purification of glycoside hydrolases using Ligation-Independent Cloning (LIC). High-throughput cloning for biophysical applications.
Hierarchical ligation-independent assembly of PCR fragments. TALEN-Based Mutagenesis of Lipoxygenase LOX3 Enhances the Storage Tolerance of Rice (Oryza sativa) Seeds.Īpplication of the Restriction-Free (RF) cloning for multicomponents assembly.ĭevelopment of new potato virus X-based vectors for gene over-expression and gene silencing assay. Synthesis of an arrayed sgRNA library targeting the human genome. Ligation-independent cloning for plant research. New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag.įast and Efficient Cloning of Cis-Regulatory Sequences for High-Throughput Yeast One-Hybrid Analyses of Transcription Factors. Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit. Screening and Production of Recombinant Human Proteins: Ligation-Independent Cloning.Ī new vector coupling ligation-independent cloning with sortase a fusion for efficient cloning and one-step purification of tag-free recombinant proteins. High-Throughput Cell-Free Screening of Eukaryotic Membrane Proteins in Lipidic Mimetics.Įfficient and high-throughput pseudorecombinant-chimeric Cucumber mosaic virus-based VIGS in maize.Ĭonstruction of a cDNA expression library in a binary vector using a nicking enzyme.
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